EFFECT OF THE SHIFTING OF DIURNAL GEOMAGNETIC VARIATION
ON THE ACTIVITY AND SENSITIVITY OF ROACH UNDERYEARLINGS'
DIGESTIVE GLYCOSIDASES TO HEAVY METALS

1 Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Yaroslavl region, Russia

2 Demidov Yaroslavl State University, Yaroslavl, Russia

* e-mail: golovanova5353@mail.ru

Abstract. Effects of the temporal shifts of diurnal geomagnetic variation for 6 and 12 hours relative to the night-day light cycle on the activity of digestive glycosidases (maltase, amylolytic activity) and their sensitivity to the in vitro influence of heavy metal ions (Cu2+, Zn2+, Pb2+, Cd2+) at a concentration of 25 mg/l was studied in roach Rutilus rutilus (L.) underyearlings. The amylolytic activity (the total activity of enzymes that hydrolyze starch: α-amylase, glucoamylase and maltase) was determined by the modified Nelson’s method, maltase activity by glucoseoxidase method with the help of the set for Clinical Biochemistry «Fotoglyukoza». It was found that the exposure of embryos to both 6- and 12-h shifts in diurnal geomagnetic variation reduces the activity of maltase and its sensitivity to the action of zinc and cadmium ions. Amylolytic activity was increased only in roach undeyearlings exposed to 6-h shift in diurnal geomagnetic variation as embryos. The sensitivity of starch hydrolyzing enzymes to the influence of copper (6- and 12-h shifts) and cadmium (12-h shift) increased. The decrease of enzyme activity and its increased sensitivity to the inhibitory effect of heavy metals may reduce the efficiency of carbohydrate hydrolysis in roach underyearlings. The increase of amylolytic activity can be considered as an adaptation of fish digestive system to the shift of diurnal geomagnetic variation for 6 hours relative to the night-day light cycle during the embryogenesis.

Keywords: diurnal geomagnetic variation, heavy metals, Cu, Zn, Pb, Cd, roach, digestive glycosidases.

About the authors

GOLOVANOVA Irina L. – Dr. Sci. (Biol.), chief researcher, Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences. Russia, 52742, Yaroslavl region, Nekouz district, p. Borok, IBIW RAS. E-mail: golovanova5353@mail.ru

FILIPPOV Andrey A. – Cand. Sci. (Biol.), senior researcher, Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences. Russia, 152742, Yaroslavl region, Nekouz district, p. Borok, IBIW RAS. E-mail: andron@ibiw.yaroslavl.ru

CHEBOTAREVA Yulia V. – Cand. Sci. (Biol.), senior researcher, Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences. Russia, 152742, Yaroslavl region, Nekouz district, p. Borok, IBIW RAS. E-mail: julia@ibiw.yaroslavl.ru

URVANTSEVA Galina A. – Cand. Sci. (Chem.), associate professor, Demidov Yaroslavl State University. Russia, 150003, Yaroslavl, ul. Sovetskaya, 14. E-mail: urvga@mail.ru

KRYLOV Viacheslav V. – Dr. Sci. (Biol.), leading researcher, Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences. Russia, 152742, Yaroslavl region, Nekouz district, p. Borok, IBIW RAS. E-mail: kryloff@ibiw.yaroslavl.ru

Cite this article as: Golovanovа I.L., Filippov A.A., Chebotareva Yu.V., Urvantseva G.A., Krylov V.V. Effect of the shifting of diurnal geomagnetic variation on the activity and sensitivity of roach underyearlings' digestive glycosidases to heavy metals, Geofizicheskie Protsessy i Biosfera (Geophysical Processes and Biosphere), 2019, vol. 18, no. 4, pp. 49–55 (in Russian). https://doi.org/10.21455/gpb2019.4-5

English version: Izvestiya, Atmospheric and Oceanic Physics, 2019, vol. 55, iss. 11. ISSN: 0001-4338 (Print), 1555-628X (Online). https://link.springer.com/journal/volumesAndIssues/11485