BACTERIAL
COMMUNITIES OF FROZEN QUATERNARY SEDIMENTS
OF MARINE ORIGIN ON
THE COAST OF WESTERN SPITSBERGEN
© 2021 E.S. Karaevskaya1, 2 *, N.E. Demidov1, V.S. Kazantsev3, I.M. Elizarov4, A.G. Kaloshin5, A.L. Petrov5, D.S. Karlov6, L. Schirmeister7, A.A. Belov8, S. Wetterich7
1
State
Scientific Centre of the Russian Federation «Arctic and
Antarctic Research Institute»,
St. Petersburg, Russia
2 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia
3 Obukhov Institute of Atmospheric Physics, Russian Academy of Sciences, Moscow, Russia
4 Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences, Vinogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia
5
Scientific-Analitical
Centre, Aprelevka Branch of the
All-Russian
Research Geological
Oil Institute, Moscow region, Aprelevka,
Russia
6 All-Russian Research Institute for Agricultural Microbiology, St. Petersburg; Pushkin, Russia
7
Alfred
Wegener Institute, Helmholtz Center for Polar and Marine Research,
Department
of Permafrost Research, Potsdam, Germany
8 Lomonosov Moscow State University, Faculty of Soil Science, Moscow, Russia
Abstract. The bacterial composition of permafrost samples taken during drilling of frozen marine sediments in the area of the Barentsburg coal mine on the east coast of Grønfjord Bay of Western Spitsbergen has been studied. The study was based on the analysis of the V4 region of the 16S rRNA gene, carried out using next generation sequencing, as well as using classical microbiological methods (direct luminescence microscopy and aerobic cultivation).The total cell number in permafrost samples ranges from 6.73±0.73106 to 3.37±0.19107 cells per g. The number of cultivable aerobic bacteria in frozen samples on 1/5 TSA and R2A media ranges from 0 to 6.20±0.45104 CFU/g. Isolates of aerobic bacteria were identified by 16S rRNA gene analysis as representatives of the genera Arthrobacter, Pseudarthrobacter, Psychrobacter, and Rhodoferax. The dominant phyla of the Bacteria domain were Actinobacteria, Proteobacteria, Chloroflexi, Nitrospirae and Firmicutes. As a result of phylogenetic analysis of the dominant operational taxonomic units, representatives of methane-oxidizing, sulfate-reducing bacteria, as well as heterotrophic bacteria involved in the transformation of organic matter were found.
Keywords: Western Spitsbergen, permafrost, marine sediments, cultivable aerobic bacteria, V4 region of 16S rRNA, methane.
About the authors
KARAEVSKAYA Ekaterina Sergeevna – State Scientific Centre of the Russian Federation «Arctic and Antarctic Research Institute». Russia, 199397, St. Petersburg, Bering st., 38; Vavilov Institute of General Genetics, Russian Academy of Sciences. Russia, 119991, Moscow, Gubkina st., 3. E-mail: katya_k_s@mail.ru
DEMIDOV Nikita Eduardovich – State Scientific Centre of the Russian Federation «Arctic and Antarctic Research Institute». Russia, 199397, St. Petersburg, Bering st., 38. E-mail: nikdemidov@mail.ru
KAZANTSEV Vladimir Sergeevich – Obukhov Institute of Atmospheric Physics, Russian Academy of Sciences. Russia, 119017, Moscow, Pyzhyovskiy alleyway, 3. E-mail: kazantsev@ifaran.ru
ELIZAROV Ivan Mikhailovich – Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences, Vinogradsky Institute of Microbiology, Russian Academy of Sciences. Russia, 117312, Moscow, av. 60 Anniversary of October, 2-2. E-mail: ivan.elizarov@gmail.com
KALOSHIN Alexander Grigorievich – Scientific-Analitical Centre, Aprelevka Branch of the All-Russian Research Geological Oil Institute. Russia, 143360, Moscow region, Aprelevka, 1st Ketritsa st., 1. E-mail: ???
PETROV Alexander Leonidovich – Scientific-Analitical Centre, Aprelevka Branch of the All-Russian Research Geological Oil Institute. Russia, 143360, Moscow region, Aprelevka, 1st Ketritsa st., 1. E-mail: a.petrov@vnigni.ru
KARLOV Denis Sergeevich – All-Russian Research Institute for Agricultural Microbiology. Russia, 196608, St. Petersburg; Pushkin, Podbelskogo highway, 3. E-mail: deniskarlov23@gmail.com
SCHIRRMEISTER Lutz – Alfred Wegener Institute, Helmholtz Center for Polar and Marine Research, Department of Permafrost Research. Germany, 14473, Potsdam, Telegrafenberg A45. E-mail: lutz.schirrmeister@awi.de
BELOV Andrey Antonovich – Lomonosov Moscow State University, Faculty of Soil Science. Russia, 119991, Moscow, Leninskie Gory, 1. E-mail: and.ant.be@gmail.com
WETTERICH Sebastian – Alfred Wegener Institute, Helmholtz Center for Polar and Marine Research, Department of Permafrost Research. Germany, 14473, Potsdam, Telegrafenberg A45. E-mail: sebastian.wetterich@awi.de
Cite this article as: Karaevskaya E.S., Demidov N.E., Kazantsev V.S., Elizarov I.M., Kaloshin A.G., Petrov A.L., Karlov D.S., Schirrmeister L., Belov A.A., Wetterich S. Bacterial communities of frozen quaternary sediments of marine origin on the coast of Western Spitsbergen, Geofizicheskie Protsessy i Biosfera (Geophysical Processes and Biosphere), 2021, vol. 20, no. 2, pp. 75–98 (in Russian). https://doi.org/10.21455/gpb2021.2-5
English version: Izvestiya, Atmospheric and Oceanic Physics, 2021, vol. 57, iss. 8. ISSN: 0001-4338 (Print), 1555-628X (Online). https://link.springer.com/journal/volumesAndIssues/11485